Original articles

Vol. 116: Issue 5 - October 2024

RNA-Based Next-Generation Sequencing in Non-Small Cell Lung Cancer patients: data from Campania, Italy

Authors

Pasquale Pisapia , Antonino Iaccarino , Caterina De Luca , Francesco Pepe , Gianluca Russo , Mariantonia Nacchio , Francesca Ambrosio , Roberto Bianco , Severo Campione , Alessandro Caputo , Pietro Carotenuto , Antonio D'Antonio , Maria D'Armiento , Vincenzo Damiano , Bruno Daniele , Giovanni De Chiara , Marco De Felice , Luigi Della Gravara , Teresa Fabozzi , Salvatore Feliciano , Cesare Gridelli , Elia Guadagno , Gennaro Ilardi , Davide Leopardo , Annamaria Libroia , Paolo Maione , Floriana Morgillo , Jessica Orefice , Luigi Panico , Danilo Rocco , Alberto Servetto , Silvia Varricchio , Pio Zeppa , Elena Vigliar , Claudio Bellevicine , Giancarlo Troncone , Umberto Malapelle

Key words: NSCLC, NGS, predictive molecular pathology, molecular oncology, RNA-based biomarkers
DOI: 10.32074/1591-951X-1015
Publication Date: 2024-11-29

Abstract

Objective. ALK, ROS1, NTRK, and RET gene fusions and MET exon 14 skipping alterations represent fundamental predictive biomarkers for advanced non-small cell lung cancer (NSCLC) patients to ensure the best treatment choice. In this scenario, RNA-based NGS approach has emerged as an extremely useful tool for detecting these alterations. In this study, we report our NGS molecular records on ALK, ROS1, NTRK, and RET gene fusions and MET exon 14 skipping alterations detected by using a narrow RNA-based NGS panel, namely SiRe fusion.

Methods. We retrospectively reviewed data on 201 advanced stage NSCLC patients who were referred to our laboratory for RNA-based molecular evaluation of ALK, ROS1, RET, NTRK gene rearrangements as well as MET exon 14 skipping.

Results. Overall, 23 (11.4%) positive cases were retrieved. Regarding molecular assessment, 11 (5.5%), 2 (1.0%), 9 (4.5%), and 1 (0.5%) out of 201 harbored an ALK, ROS1, RET gene rearrangement, or MET exon 14 skipping, respectively.

Conclusions. In this study, we provide real-world experience on RNA-based NGS analysis in patients with advanced stage NSCLC.

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Authors

Pasquale Pisapia - Department of Public Health, University of Naples Federico II, Naples, Italy

Antonino Iaccarino - Department of Public Health, University of Naples Federico II, Naples, Italy

Caterina De Luca - Department of Public Health, University of Naples Federico II, Naples, Italy

Francesco Pepe - Department of Public Health, University of Naples Federico II, Naples, Italy

Gianluca Russo - Department of Public Health, University of Naples Federico II, Naples, Italy

Mariantonia Nacchio - Department of Public Health, University of Naples Federico II, Naples, Italy

Francesca Ambrosio - Oncology Unit, A.O.R.N. Cardarelli, Hospital Antonio Cardarelli, Naples, Italy

Roberto Bianco - Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy

Severo Campione - Pathology Unit, A.O.R.N. Cardarelli, Hospital Antonio Cardarelli, Naples, Italy

Alessandro Caputo - Department of Pathology, University Hospital of Salerno, Salerno, Italy

Pietro Carotenuto - Department of Translational Medical Science, Medical Genetics, University of Naples Federico II, Naples, Italy; TIGEM, Telethon Institute of Genetics and Medicine, Pozzuoli, Naples, Italy

Antonio D'Antonio - Pathology Unit, Ospedale del Mare, Naples, Italy

Maria D'Armiento - Department of Public Health, University of Naples Federico II, Naples, Italy

Vincenzo Damiano - Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy

Bruno Daniele - Department of Translational Medical Science, Medical Genetics, University of Naples Federico II, Naples, Italy

Giovanni De Chiara - Division of Pathology, “S.G. Moscati” Hospital, Avellino, Italy

Marco De Felice - edical Oncology Unit, Ospedale Ave Gratia Plena, Piedimonte Matese, Caserta, Italy

Luigi Della Gravara - Department of Precision Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Naples, Italy

Teresa Fabozzi - Oncology Unit, Ospedale del Mare, Naples, Italy

Salvatore Feliciano - Medical Oncology Unit, Ospedale Ave Gratia Plena, San Felice a Cancello, ASL Caserta, Caserta, Italy

Cesare Gridelli - Division of Medical Oncology, “S.G. Moscati” Hospital, Avellino, Italy

Elia Guadagno - Pathology Unit, A.O.R.N. Cardarelli, Hospital Antonio Cardarelli, Naples, Italy

Gennaro Ilardi - Department of Advanced Biomedical Sciences, University of Naples Federico II, Naples, Italy

Davide Leopardo - Department of Oncology, A.O.R.N. Sant’Anna e San Sebastiano, Caserta, Italy

Annamaria Libroia - Oncology Unit, “Andrea Tortora” Hospital, ASL Salerno, Pagani, Italy

Paolo Maione - Division of Medical Oncology, “S.G. Moscati” Hospital, Avellino, Italy

Floriana Morgillo - Department of Precision Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Naples, Italy

Jessica Orefice - Oncology Unit, Ospedale del Mare, Naples, Italy

Luigi Panico - Department of Pathology, A.O.R.N. dei Colli Monaldi, Naples, Italy

Danilo Rocco - Department of Pulmonary Oncology, A.O.R.N. dei Colli Monaldi, Naples, Italy

Alberto Servetto - Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy

Silvia Varricchio - Department of Advanced Biomedical Sciences, University of Naples Federico II, Naples, Italy

Pio Zeppa - Department of Pathology, University Hospital of Salerno, Salerno, Italy

Elena Vigliar - Department of Public Health, University of Naples Federico II, Naples, Italy

Claudio Bellevicine - Department of Public Health, University of Naples Federico II, Naples, Italy

Giancarlo Troncone - Department of Public Health, University of Naples Federico II, Naples, Italy

Umberto Malapelle - Department of Public Health, University of Naples Federico II, Naples, Italy

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Copyright (c) 2024 Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology

How to Cite
Pisapia, P., Iaccarino, A., De Luca, C., Pepe, F. ., Russo, G., Nacchio, M., Ambrosio, F., Bianco, R., Campione, S., Caputo, A., Carotenuto, P., D’Antonio, A., D’Armiento, M., Damiano, V., Daniele, B., De Chiara, G., De Felice, M., Della Gravara, L., Fabozzi, T., Feliciano, S., Gridelli, C., Guadagno, E., Ilardi, G., Leopardo, D., Libroia, A., Maione, P., Morgillo, F., Orefice, J., Panico, L., Rocco, D., Servetto, A., Varricchio, S., Zeppa, P., Vigliar, E., Bellevicine, C., Troncone, G., & Malapelle, U. (2024). RNA-Based Next-Generation Sequencing in Non-Small Cell Lung Cancer patients: data from Campania, Italy. Pathologica - Journal of the Italian Society of Anatomic Pathology and Diagnostic Cytopathology, 116(5). https://doi.org/10.32074/1591-951X-1015
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